(2) Never put oil or grease on the valves of cylinders or pressure regulators.
(3) Do not exceed the pressure or temperature that has been designated as the safe upper limit for the
apparatus or equipment being used. Do not use cylinder without a regulator.
(4) Do not use faulty copper or rubber tubing when performing operations requiring pressure or vacuum.
(5) Make sure that glass vacuum apparatus is properly shielded when it is in use.
(6) Always wear goggles when opening air valves that are close to the face.
(7) Make sure that chemical containers having vent caps are inspected and that containers which do not have
vent caps are vented periodically.
(8) Keep containers of volatile liquids as cool as possible. Exercise caution in releasing any pressure which
may have formed in the container; always release the pressure gradually. Remove caps or stoppers periodically to vent
the vapor. The practice of venting containers of volatile liquids does not apply to those samples collected for vapor
(9) Vent separatory funnels frequently when shaking volatile liquids. Always wrap the funnel with a rag when
shaking an extremely volatile liquid.
(10) Store compressed gas cylinders in ventilated area away from heat or ignition sources.
f. Controlling Fumes.
Before proceeding, keep in mind the WARNINGS in the front of this manual.
(1) Make certain the laboratory is properly ventilated and that fume hoods are operating properly.
(2) Always evaporate fuels in a fume hood.
(3) When using benzene, aniline, bromine water, or other materials producing toxic vapors, always work in a
(4) If any material is spilled which gives off toxic fumes, all personnel should leave the area immediately and
return only after the area has been adequately ventilated, or after suitable breathing equipment has been obtained.