(c) To clear the valve of particles of dust or dirt that might enter pressure regulator, open cylinder valve
one quarter turn counterclockwise, and close immediately.
(d) Attach oxygen gas pressure regulator to cylinder valve outlet and to oxygen line of equipment.
(e) Turn pressure regulator adjusting screw counterclockwise until pressure regulator valve is fully open,
and slowly open cylinder valve to full OPEN position.
(f) Turn pressure regulator adjusting screw clockwise until desired working pressure is reached.
(g) To shut down, close cylinder valve. If pressure regulator is to be removed, release all oxygen from
regulator and disconnect. Replace outlet connection cap and valve protection cap.
(2) Liquified Petro Propane Gas Cylinders.
(a) Place cylinder or cylinders firmly in position. Normally cylinders should be located outside of the
(b) Operate cylinder as in (1) above, using propane gas pressure regulator rather than oxygen gas
pressure regulator. Connect regulator to gas service lines of the laboratory.
(3) Carbon Dioxide Gas Cylinder.
(a) Remove valve protection cap and outlet connection cap.
Connect cylinder to equipment to be
charged with carbon dioxide gas, and slowly open the cylinder valve.
(b) To shut down carbon dioxide cylinder, close cylinder valve; disconnect cylinder from equipment, and
replace outlet connection cap and valve protection cap.
(c) Replace cylinder in proper position in storage.
(4) Refrigerant -12 Gas Cylinder.
(a) Remove valve protection cap and outlet connection cap; connect outlet connection to equipment to
be charged, and slowly open cylinder valve.
(b) Close cylinder valve as soon as equipment is charged or cylinder is empty; disconnect cylinder from
equipment, and replace outlet connection cap and valve protection cap.